Differences in small molecule binding for human to rat orthologs.

<p>(a) Summary scatter plot. Positions along axes report the median binding affinity of all compounds against the human target (x-axis) and its ortholog in rat. Points are scaled according to the number of different compounds tested against a pair of orthologs. (b) Scatter plot of individual compound binding affinities for the human targets (x-axis) and their orthologs in rat (y-axis). (c) Scatter plot of the affinities observed when comparing results from different assays of the same compound and target (1500 human targets and 1500 rat targets). (d) Distribution of differences in binding affinity between the human target and its ortholog in rat. Positive values indicate higher affinity for the human target and vice versa. (e) Distribution of the differences observed when comparing binding affinities for the same compound and target in different assays.