Western blot and lectin blot PC analysis.

<p>(A) Western blotting analysis of kidney lysates, using an anti-PC antibody. PC bands in wt and ht mice were about 140–150 kDa (arrowheads), while those in mt mice showed a shift to higher molecular weights (bracketed). (B) Neuraminidase treatment shifted the PC bands in wt mice to higher molecular weights similar to those seen in untreated mt mice. (C to E) Glycan analysis of PC by lectin blotting. After immunoprecipitation with an anti-PC antibody, PC bands were detected by PC (C), RCA-I (D), and PNA (E).