Impact of concentration of universal primer on singlet PCR.

<p>Lane 0, negative control without template; lanes 1, 2, 3, 4, amplicon fragments by compound specific primer pair Lec-118-F/R at the concentration of 500 nmol L⁻¹, 50 nmol L⁻¹, 25 nmol L⁻¹, 5 nmol L⁻¹ respectively; lanes 5, 6, 7, 8, amplicon fragments by UP (500 nmol L⁻¹) and compound specific primer Lec-118-F/R at a series concentrations of 500 nmol L⁻¹, 50 nmol L⁻¹, 25 nmol L⁻¹,5 nmol L⁻¹; lane M, 100 bp DNA Marker.