Neural cultures derived from KLC1-suppressed hESC have reduced neural microtubule-associated markers.

<p>(A) Cultures were harvested at seven weeks <i>in vitro</i> and equal protein from mouse PA6 feeders cultured with control, <i>shKLC1-1</i> or no hESC (PA6 feeder cells lane) were analyzed by Western blotting for actin, GAPDH and SOD1. Note that unlike Actin, mouse and human GAPDH and SOD1 have different electrophoretic mobilities (arrows). (B–C) Control or <i>shKLC1-1</i> hESC were cultured for seven weeks with PA6 feeder cells and then harvested. Equal protein from control and <i>shKLC1-1</i> cultures was analyzed by Western blotting. (B) Representative immunoblots of Actin, NSE, GFAP, α-Tubulin, β-III-Tubulin, MAP2, pNF-H, pNF-M, Tau and pTau. (C) Quantification of protein levels relative to control and normalized to Actin. Based on n = 6 wells each *p<0.05, **p<0.01, ***p<0.001 by 2 tailed t-test compared to control.