Cellular composition of CSs from mouse hearts.

<p>(A) Flow cytometric analysis of Sca-1, CD45 and c-kit expression in disaggregated CS cells. Typical results are shown (N = 6). (B, C) Bar graph showing the profile of progenitor cell markers in CSs by FACS (N = 6). (D) Immunocytochemical staining demonstrates that CS cells express Isl1, Nkx2-5 and GATA4. Arrows point to positive staining cells (red). Nuclei stained with DAPI. Scale bar = 35 µm and 15 µm (NKx2-5). (E) Bar graph shows the profile of Isl1, Nkx2-5, GATA4 positive cells in CSs by immunocytochemical staining. Data in Figure shown as mean±SEM.