Mitotic translocating radial glial (tRG) cells in rodent cortex express Pax6.

<p>(<b>a</b>) Example of a mitotic tRG cell labeled with 4A4 (green). The pial fiber of the dividing cell is seen coursing to the pial surface. All mitotic tRG cells coexpressed Pax6 (red). DAPI stained nuclei are shown (blue). (<b>b</b>) An example of a GFP+ tRG cell (green) labeled through in utero retroviral injection at E16. The tRG cell (arrowhead) expresses Pax6 (red). A daughter cell can be seen migrating along the pial fiber (arrow). All tRG cells labeled with retroviral injections expressed Pax6. (<b>c</b>) tRG cells labeled in the E18 mouse through in utero electroporation with a GFP expressing plasmid (green) expressed both Pax6 (red) and Sox2 (blue). Left panel shows a low magnification merged image of the tRG cell. Middle images show high magnification individual images of GFP (green), Pax6 (red) and Sox2 (blue). Right image shows high magnification merged image of GFP (green), Pax6 (red) and Sox2 (blue). All GFP+ cells with tRG morphology in mouse were Pax6+ and Sox2+.