Tau-1 and Tbr2 staining distinguishes the inner subventricular zone (iSVZ) from the outer SVZ (oSVZ).

<p>(<b>a</b>) A coronal section from E80 macaque somatosensory cortex immunostained for Tau-1 (green), Tbr2 (red), and counterstained with DAPI (blue). Tau-1 staining produces dense labeling in the cortical plate, striated staining in the outer subventricular zone (oSVZ) and an absence of label in the inner SVZ (iSVZ). The striated appearance of Tau-1 staining in the oSVZ complements the striated pattern of the oSVZ that is seen in Nissl or DAPI stained tissue. (<b>b</b>) Inset from (<b>a</b>) showing the pattern of cell density (DAPI, blue), Tbr2 staining (red), and Tau-1 staining (green). The Tau-free zone corresponds to the cell dense iSVZ/VZ visualized in DAPI staining (blue) and where the dense inner band of Tbr2+ cells (red) is located. The boundary between the Tau-free and the Tau-striated zone delineates the boundary between the iSVZ and oSVZ where the diffuse outer band of Tbr2+ cells is located. (<b>c</b>) Tau-1 and Tbr2 immunostaining produces the same pattern in the E20 rat. The Tau-free zone corresponds to the cell dense VZ/iSVZ visualized in DAPI staining (blue) and where the dense inner band of Tbr2+ cells (red) is located. The boundary between the Tau-free and the Tau-striated zone delineates the boundary between the iSVZ and oSVZ where diffuse Tbr2+ cells are located. (<b>d</b>) Tau-1 and Tbr2 immunostaining produces the same pattern in the P2 ferret. The Tau-free zone corresponds to the cell dense VZ/iSVZ visualized in DAPI staining (blue) and where the dense inner band of Tbr2+ cells (red) is located. The boundary between the Tau-free and the Tau-striated zone delineates the boundary between the iSVZ and oSVZ where diffuse Tbr2+ cells are located.