Western blot analysis of PRC2 association with chromatin during MCMV infection.

<p>(A) Validation of chromatin fractionations. Mouse fibroblasts were fractionated as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029410#s4" target="_blank">Materials and Methods</a>. 25 µg of protein from the soluble (S) and chromatin (C) fractions were probed for the chromatin-associated proteins SUZ12, HDAC1 and HP1. The blot was then stripped and re-probed for the ER-associated protein GRP78. (B) Chromatin fractions prepared from vehicle-treated (DMSO), cycloheximide (100 µg/ml) or phosphonoacetic acid (200 µg/ml) treated, mock-infected (M) or MCMV-infected fibroblasts at the indicated times. Blots were probed with antibodies against the PRC2 components EZH2 and SUZ12, or HDAC1 and HP1 for a loading control and fractionation quality control, respectively.