Co-immunofluorescence assay for PRC2 enrichment within MCMV replication compartments.

<p>At 18 hpi, mock-infected or MCMV-infected fibroblasts were fixed and incubated with antibodies against M44 and (A) EZH2, (B) SUZ12 or (C) H3K27me3. (D) Purified rabbit IgG served as an isotype control. The first column of panels displays DAPI staining for nuclei. The second column of panels displays M44 staining, marking MCMV replication compartments. The third column of panels displays staining EZH2, SUZ12, H3K27me3 or isotype IgG. The fourth column of panels displays a merged image of the first three channels. The fifth column of panels displays a high-magnification image of the merged image from the fourth column. All images are 40×, unless otherwise indicated.