Arrested wild-type L1 larvae incorporate medium ethanol into stearic and palmitic acids.

<p>The isotopic distribution of stearic acid methyl ester and palmitic acid methyl ester from L1 larvae incubated for 7 days in 1 mM fully-deuterated ethanol is shown for the non-deuterated “sharp” peak (panels A and C) and for the earlier-eluting broad deuterated peak (panels B and D) (compare with <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029984#pone-0029984-g008" target="_blank">Figure 8</a>, panel B). Isotopic analysis of the broad peaks shows deuterium incorporation into stearic acid methyl ester that results in addition of 4–10 mass units (panel B) not seen in non-deuterated peak (panel A). Similarly, the earlier-eluting peak for palmitic acid methyl ester shows deuterium incorporation that results in addition of 4–7 mass units (panel D) not seen in the non-deuterated material (panel C).