RKIP depletion induces hydrogen peroxide and chemotherapeutic resistance.

<p>A, WST assay showing enhanced survival of RKIP depleted HEK-499 compared to control cells with increasing hydrogen peroxide concentration. B, MTT assay/dose response curve depicting the chemosensitivity of HEK-293 and HEK-499 after treatment with various Cisplatin concentrations for 16 hours and (C) for 60 hours. D, Early apoptosis estimation using Anexin V of HEK-293 and 499 cells after treatment with 10 µg/ml Cisplatin for 16 hours demonstrating significant resistance of HEK-499 cells to apoptotic death. Asterisks indicate statistical significance (p<0.05 compared to equivalent controls).