<i>FAD2</i> gene mutation and fatty acid analyses in <i>fad2</i>.

<p>(A) Intron-exon organization of the Arabidopsis <i>FAD2</i> gene (not to scale). The mutation site was indicated. Solid boxes and lines represent exons and introns, respectively. (B) The amino acid mutation site in the predicted topology of FAD2 protein. Three His boxes (black ellipses) oriented on the cytosolic side of the membrane coordinate two iron atoms (gray circles) at the active site of the protein. The mutation site was indicated (from A₁₀₄ to T₁₀₄). (C) Fatty acid analyses of Col-0, <i>fad2</i> and <i>fad2</i> mutant complemented with the functional wild-type <i>FAD2</i> or the mutated <i>FAD2Δ</i> gene. Col-0, wild-type Columbia; <i>fad2</i>, <i>FAD2</i> mutant; <i>fad2/FAD2-1</i> and <i>fad2/FAD2-2</i>, two transgenic <i>fad2</i> lines complemented with the functional Col-0 <i>FAD2</i> gene; <i>fad2/FAD2Δ-1</i> and <i>fad2/FAD2Δ-2</i>, two transgenic <i>fad2</i> lines complemented with the mutated <i>FAD2Δ</i> gene. Results are presented as means and standard errors from three independent experiments. ** indicates significant difference in comparison to Col-0 at P<0.01 (Student's t-test).