Technical and biological variation in the faecal metaproteomic analysis of subjects A, B and C.

<p>Sample similarities are expressed as Pearson correlations, which were calculated for peptide feature intensities between different sample preparation replicates (experimental), different time point (temporal), and subjects (A, B, C) (ND: not determined; SD: standard deviation; n: number of comparisons). Column “A, B” presents data from an experiment in which the metaproteome of subjects A and B time point one were analysed and column “A, B, C” shows data of a repetition of those samples and an addition of a second time point and subject C. For detailed experimental setup see “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029913#s4" target="_blank">Material and Methods</a>” <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029913#pone.0029913.s001" target="_blank">Figure S1</a>.