The response to DNT and the basal level of fluorescence.

<p>The diagram shows the mean response to DNT (A) and the mean basal level of fluorescence (B) for randomly selected clones from consecutive steps during the sorting procedure until the first sorting of library 5. The first number indicates the library, and the second number indicates the sorting step for that library. A) The response to DNT was measured for 5–10 clones for each sorting step. For each clone the response was calculated as the ratio between the mean fluorescence of 10000 cells grown in the presence of 500 µM DNT and the mean fluorescence of 10000 cells grown in the presence of the solvent DMSO. Shown in each bar is the mean for all the analyzed clones in that sorting step and in error bars are the standard deviation of the DNT response for these clones. As a comparison the mean response to DNT for wt DntR is shown with the standard deviation based on five independent data sets. B) The basal level of fluorescence is calculated as the ratio between the fluorescence of the population with DMSO added compared to the fluorescence of a WT DntR control with DMSO added for the same clones as in A). Clones were also analyzed for library 5, but since these were grown in M9* and first subjected to a screen on plate instead of randomly selected, there is no comparable data available.