Effects of Pmk1 signaling on stress granule assembly.

<p>(A) Effects of Pmk1 deletion on SG assembly. Wild-type cells and Pmk1-deleted cells expressing Nrd1-GFP were grown in YES at 27°C (untreated). Cells were treated with heat shock (42°C for 4 min, or 20 min). Lower panel: Graphs showing the number of stress granules per cell from each strain. (B) Effects of Pmk1 hyperactivation on SG assembly. Wild-type cells expressing Nrd1-GFP were transformed with the constitutively active Pek1^DD or the control vector, and grown in EMM at 27°C (untreated). Cells underwent heat shock at 42°C for 3, or 20 min. Graphs showing the number of stress granules per cell from each strain. The inset is a magnification of the results obtained in each strain at 0 min and 3 min.