Relationship between relative differences in gene expression and type of vaccine for differentially regulated genes.

<p>Data from all four experiments using the full panel of vaccine candidates (<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002494#ppat.1002494.s006" target="_blank">Table S2</a>, experiments 4 – 7) are used to provide a graphic representation of the relationship between type of vaccine and relative gene expression. All data were normalized in relationship to endogenous GAPDH standard curves, and values shown are median of the fold differences (ΔΔCt) in relationship to naive cells for each type of vaccine, as indicated. Panel A depicts the genes designated as Group 1 in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002494#ppat-1002494-t001" target="_blank">Table 1</a> (all significant, p<0.0001); Panel B depicts the genes designated as Group 2 in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002494#ppat-1002494-t001" target="_blank">Table 1</a> (all significant, p<0.0001); Panel C depicts the remaining up-regulated genes not included in Groups 1 or 2 of <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002494#ppat-1002494-t001" target="_blank">Table 1</a> (IL-17A, IL-23a, CCR5, and STAT-1 significant with p<0.05, but IL-13 not significant; see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002494#ppat.1002494.s008" target="_blank">Table S4</a> for exact p values); and Panel D depicts down-regulated genes (all non-significant; see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002494#ppat.1002494.s008" target="_blank">Table S4</a> for p values). The corresponding legends are shown immediately above or immediately below each panel.