Linear standard curves for the PC gene and Cm insertion.

a<p><i>y</i> = C_t value (PCR cycle value at the fluorescence threshold of 0.1 for PC gene and 0.08 for Cm insertion), x = amount of template DNA (expressed as cell number equivalents),</p>b<p>Amplification efficiencies (E) were calculated as follows E = (10^−1/slope−1)×100.