Identification of critical residues for exon 5 inclusion into mA3 mRNA.

<p>(A) Polymorphic nucleotides indicated with the shades were exchanged at the boxed sites between the B6 and BALB/c alleles. The numbers 14, 88, 153, and 163 above the shown nucleotide sequence are base numbers from the first nucleotide of exon 5. (B and C) The results of splicing assays performed with the plasmids depicted in (A). The predicted splicing product is shown on the right side of each panel. Band intensities are averages of 3 independent RT-PCR reactions with SD.