MCSF-1 and MMP-9 decrease with EGFR inhibition.

<p>(A) anti-VEGF probed western blot for MDA-231, shControl, and shEGFR-MDA-231 extracts, β-tubulin used for loading control. (B) Media was harvested from shControl or shEGFR-MDA-231 cells and analyzed for MCSF-1 by ELISA, *p<0.05. Measurements were obtained from two separate cultures, and performed in triplicate. (C) MDA-231, shControl, SUM149, or NS2TA1 cells were treated with the tyrosine kinase inhibitor PD153035 (10 µg/mL) compound for 6 hours or PAR34 (10 µg/mL) for 24 hours before media harvest for MCSF-1 ELISA, *p<0.05 and **p<0.01. Measurements were obtained from two separate cultures, and performed in triplicate. (D) anti-MMP9 antibody probed western blots for shControl, MDA-231, SUM149, or NS2TA1 cell extracts treated with PD153035 (10 µg/mL) compound for 6 hours or PAR34 (10 µg/mL) for 24 hours. shEGFR-MDA-231 cells were untreated. anti-β-tubulin used as loading control.