Comparison of the inhibitory effects of 25 µM Kae and Dai on JEV E, NS1 and NS1' protein expression at 48 h.

<p>(A) Histogram of JEV E, NS1 and NS1' protein expression levels in cells infected and then treated with Kae or Dai. (B) Histogram of viral E, NS nd NS1' protein expression levels in cells pretreated with Kae or Dai before infection. (C) Representative western blots of E, NS1 and NS1' protein expression. The E, NS1 and NS1' protein level in JEV-infected cells (mock infection) was defined as 100%. The amount of E, NS1 and NS1' proteins was normalized to the amount of cellular β-actin. Mock infection: infection alone without compound treatment; pretreatment: cell monolayers pretreated with Kae or Dai for 2 h, and then infected for 48 h; preinfection: cell monolayers infected with 0.1 MOI of JEV for 2 h and then treated with Kae or Dai for 48 h, and 7-hydroxyflavone as treatment control. Data shown were from three independent experiments with the mean and standard errors.