Primary tumors and metastasis from HNSCC patients contain a subpopulation of CD49f^high cells.

<p>(<b>A–D</b>) Representative CD49f staining in primary tumors (A and C) and lymph node metastasis (B and D) from two different patients. CD49f^high tumor cells are marked with arrows. Note the strong cytosolic and membrane pattern of the signal (arrowhead in the, inset) and the overall stronger signal and increased frequency for CD49f in the metastatic lesions (B and D). Scale bar: 80 µm and 40 µm inset scale bar. (<b>E–F</b>) Immunofluorescence detection of CD49f in sections from human oral primary tumors (E) and metastasis (F) (PT68880). Scale bar: 60 µm. (<b>G</b>) Quantification of CD49f^high cells in sections from primary tumors and lymph node metastasis. Columns represent mean of 5 different sections per sample and a minimum of 500 cells was scored per sample. p-values estimated using one-way ANOVA followed by the Bonferroni correction with two-tailed <i>P</i> values<0.05 considered significant.