Quantification of bacterial lysis.

<p>A) 10 µL of bacterial culture of GBS WT, PilB⁻ and PilA/C⁻ were stained with Syto9 and propidium iodide (PI). The bacterial suspensions were then subjected to FACS and counted using the LIVE/DEAD® <i>Bac</i>Light™ kit. B) OD_{600 nm} measurement of GBS or <i>S. aureus</i> suspensions in PBS at 37°C over 5 h. The graphic expresses the % in OD in different time points relative to the time 0. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029963#s2" target="_blank">Results</a> shown are representative of two independent experiments. *, P<0.05.