Effects of compound C on mangiferin induced FFA uptake, intracellular FFA, TG and FFA metabolism proteins expression in HepG2 cells.

<p>HepG2 cells were pretreated 1 h with compound C, an AMPK inhibitor, and then treated with 100 µmol/L mangiferin and 0.2 mmol/L OA for 24 h. OA in medium (To assess the uptake of OA) (A), intracellular OA (A), intracellular TG (A) and proteins expression of FFA metabolism including AMPK (B), ACC (B), CD36 (B), CPT1 (B) and DGAT2 (B) were determined by western blot method. The experiments were repeated 3 times. Data are presented as means ± SD (n = 3). ^*<i>P</i><0.05 and ^**<i>P</i><0.01 compared with only OA stimulation group.