Palladin and a stimulatory trigger enhance HDF cell migration, invasion, and degradation of extracellular matrix.

<p><b>A</b>) Migration across a transwell was compared for HDF transfected with empty vector (EV), WT-palladin (WT), FX-palladin (FX) when exposed to normal complete media or wounding media. Data shown is representative average ± SEM of three independent experiments. (t-test, *, p-value<0.05) <b>B</b>) Invasion across a matrigel-covered transwell was compared for HDF cells treated as above in (<b>A</b>) when exposed to complete media, wounding media, or conditioned media from Panc-1 cells. Data shown is representative average ± SEM of three independent experiments. (t-test, **, p-value<0.01) <b>C</b>) HDF cells were transfected as above in (<b>A</b>) and plated onto coverslips coated with Texas Red-labeled gelatin. Representative images taken via IF are shown. DAPI (blue) was used to visualize nuclei. <b>D</b>) Protein lysates from HDF cells transfected as above in (<b>A</b>) were tested for RhoA activity. Each sample was run in triplicate in two independent experiments. Error bars indicate SD. (t-test, **, p-value<0.01) <b>E</b>) Proliferation assay of HDF cells transfected as above in (<b>A</b>). Data indicates mean ± SD for three independent wells.