Knocking out mitochondrial encoded reverse transcriptase activity results in increased transcript levels of nuclear genes that are involved in Mito-nDNA interactions.

<p>A) Nuclear encoded <i>MSY1</i> transcript levels were determined by qRT-PCR in WT (strain 161-U7), 161-U7 GII-0 (lacks both the mitochondrial group II introns and the <i>COX1</i> interacting region; <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030943#pone-0030943-g004" target="_blank">Figure 4A</a>), and 161-U7 GII-0 a15γ (contains the interacting region and lacks the group II introns; <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030943#pone-0030943-g004" target="_blank">Figure 4A</a>) cells. B) Nuclear encoded <i>RSM7</i> transcript levels were determined by qRT-PCR in: WT (strain 161-U7); 161-U7 GII-0; and 161-U7 GII-0 a15γ cells. Neither 161-U7 GII-0 nor 161-U7 GII-0 a15γ has any alteration within the <i>Q0182</i> open reading frame. C) Deletion of <i>MRS1</i> (BY4741 Δ<i>mrs1</i>), a nuclear gene involved in splicing mitochondrial type-I introns, has no effect on i) <i>MSY1</i> or ii) <i>RSM7</i> transcript levels. All transcript levels were standardized to nuclear <i>ACT1</i> and expressed as percentage of wild-type (set at 100%) +/− standard error of the mean (n = 2).