Functional manipulation of miR-16 impacts LPS-stimulated expression of IL-6, IL-8, and IL-1α.

<p>(A) Multiplex bead array analysis of IL-6, IL-8, and IL-1α proteins in H69 cells transfected with pre-control or miR-16 precursor for 48 h, followed by exposure to LPS for 12 h. Data shown are averages of three independent experiments. ∗, p<0.05 <i>t</i>-test vs. the non-treated control. (B) and (C) Effects of miR-16 precursor on LPS-induced upregulation of mRNAs for IL-8, IL-6, and IL-1α in H69 (B) and U937 (C) cells. mRNA levels of IL-8, IL-6, and IL-1α were measured by real-time PCR in cells transfected with the pre-control or miR-16 precursor for 48 h, followed by exposure to LPS for 6 h. (D) Effects of anti-miR-16 on LPS-induced upregulation of mRNAs for IL-8, IL-6, and IL-1α in H69 cells. Real-time PCR analysis was used to assess IL-8, IL-6, and IL-1α mRNA levels in H69 cells transfected with anti-control or anti-miR-16 for 48 h, followed by exposure to LPS for 6 h. Data are averages of three independent experiments. ∗, p<0.05 <i>t-</i>test vs. the controls.