LPS stimulation decreases SMRT expression by upregulating miR-16.

<p>(A) and (B) H69 and U937 cells were exposed to LPS for up to 24 h, followed by Western blotting for the SMRT protein and real-time PCR analysis for SMRT mRNA. Downregulation of the SMRT protein (A), but not SMRT mRNA level (B), was detected in both H69 and U937 cells following LPS stimulation. (C) H69 cells were treated with LPS for 24 h in the presence or absence of SC-514 (100 µM), followed by Western blot for SMRT protein. SC-514 showed no significant effects on LPS<i>-</i>induced decrease of SMRT. (D) Effects of anti-miR-16 on LPS-induced downregulation of the SMRT protein in H69 and U937 cells. Cells were transfected with anti-control (30 nM) or anti-miR-16 (30 nM) for 24 h and cultured for an additional 24 h in the presence or absence of LPS (1 µg/ml). Representative blots from at least three independent experiments are shown. β-actin was blotted as a loading control.