Genotypic and PFGE/MLVA Analyses of <em>Vibrio cholerae</em> O1: Geographical Spread and Temporal Changes during the 2007–2010 Cholera Outbreaks in Thailand

<div><h3>Background</h3><p><em>Vibrio cholerae</em> O1 El Tor dominated the seventh cholera pandemic which occurred in the 1960s. For two decades, variants of <em>V. cholerae</em> O1 El Tor that produce classical cholera toxin have emerged and spread globally, replacing the prototypic El Tor biotype. This study aims to characterize <em>V. cholerae</em> O1 isolates from outbreaks in Thailand with special reference to genotypic variations over time.</p> <h3>Methods/Findings</h3><p>A total of 343 isolates of <em>V. cholerae</em> O1 from cholera outbreaks from 2007 to 2010 were investigated, and 99.4% were found to carry the classical cholera toxin B subunit (<em>ctxB</em>) and El Tor <em>rstR</em> genes. Pulsed-field gel electrophoresis (PFGE) differentiated the isolates into 10 distinct pulsotypes, clustered into two major groups, A and B, with an overall similarity of 88%. Ribotyping, multiple-locus variable-number tandem-repeat analysis (MLVA), and PCR to detect <em>Vibrio</em> seventh pandemic island II (VSP-II) related genes of randomly selected isolates from each pulsotype corresponded to the results obtained by PFGE. Epidemiological investigations revealed that MLVA type 2 was strongly associated with a cholera outbreak in northeastern Thailand in 2007, while MLVA type 7 dominated the outbreaks of the southern Gulf areas in 2009 and MLVA type 4 dominated the outbreaks of the central Gulf areas during 2009–2010. Only MLVA type 16 isolates were found in a Thai-Myanmar border area in 2010, whereas those of MLVA types 26, 39, and 41 predominated this border area in 2008. Type 39 then disappeared 1–2 years later as MLVA type 41 became prevalent. Type 41 was also found to infect an outbreak area.</p> <h3>Conclusions</h3><p>MLVA provided a high-throughput genetic typing tool for understanding the in-depth epidemiology of cholera outbreaks. Our epidemiological surveys suggest that some clones of <em>V. cholerae</em> O1 with similar but distinctive genetic traits circulate in outbreak sites, while others disappear over time.</p>