Effect of dominant negative IFI16 (dnIFI16) overexpression on Herpesvirus growth.

<p>A) Western blot analysis was carried out to detect IFI16 and V5 expression in HELFs stably transduced with the recombinant Lentivirus carrying the full-length IFI16 (wtIFI16), mutated forms of IFI16 (dnIFI16), lacking the PYD domain (ΔPYDIFI16, indicated as ΔDIFI16), the HIN-B domain (ΔHIN-BIFI16, indicated as ΔBIFI16) or expressing the LacZ transgene as negative control (LacZ). β-actin immunodetection was used to control for equal loading. B) HELFs carrying wtIFI16, ΔDIFI16, ΔBIFI16 or the control LacZ gene were infected with HCMV at the indicated MOI. Viral supernatants were collected at 96 hours post infection (hpi) and analyzed by standard plaque assay. The data shown are the average of three experiments ± SD (*p<0.05, one-way ANOVA followed by Bonferroni's post test).