Expression analyses of Aurora A and TPX2 in control and withanone- treated human breast carcinoma (MCF 7) cells.

<p>(A) RT-PCR with gene specific primers and (B) Western blotting with specific antibodies. (C) Immunolocalization of TPX2 and Aurora A in control and withanone treated MCF 7 cells showing Aurora A (red), TPX2 (green) and co-localization of the two (yellow). (D) Representative high magnification images of TPX2 and Aurora A immunostaining showing normal and disrupted mitotic spindle in control and withanone treated cells, respectively.