Tight junction networks formed by Cld3-YFP and Cld5-YFP.

<p><b>A</b>: Localization microscopy image of the region marked in the conventional wide-field fluorescence image (<b>B</b>) of YFP labeled Cld3 in HEK293 cells. More than 450,000 molecules were detected with a mean localization accuracy of ∼20 nm. The mean distance to the next neighboring molecule in the image is ∼10 nm, thus the mean effective optical resolution is only limited by the localization accuracy and yielding ∼48 nm. Magnified images of the region marked in <b>A</b> are shown in <b>D</b> and <b>E</b>. Mesh-like structures identified and analyzed by the algorithm are indicated in white. <b>C</b> represents the same region but taken from the conventional wide-field fluorescence image. <b>F</b>–<b>J</b>: Analogue visualization of Cld5-YFP expressed in HEK293 cells. Here, ∼260,000 molecules were detected with a mean localization accuracy of ∼21 nm. The mean distance to the next neighboring molecule in this image is ∼7 nm; yielding a structural resolution of ∼50 nm.