Densities of detected proteins and shape of the mesh-like structures.

<p><b>A</b>,<b>B</b>: Localization microscopy images of Cld3-YFP (<b>A</b>) and Cld5-YFP (<b>B</b>) with overlay of single molecule positions represented by red crosses. <b>C</b>: Histogram of the density of detected proteins on the strands of the mesh-like structures (indicated in <b>A</b>,<b>B</b> by orange arrows). Both protein types show similar distributions with mean values of ∼3700 proteins/µm². The standard deviation of the distribution for Cld3 is 760 proteins/µm². The distribution for Cld5 is wider and provides a standard deviation of 900 proteins/µm². <b>D</b>: Histogram of the density of detected proteins beside the strands of the meshes (indicated in <b>A</b>,<b>B</b> by green arrows). Here, the distribution for Cld3 and Cld5 are very similar, too (mean values: ∼780 proteins/µm² with STDs of ∼640 proteins/µm²). For many meshes (especially the very small ones) no proteins could be detected on their inside. These are not considered in the histograms. <b>E</b>: Histograms of the minimum diameter divided by its maximum perpendicular diameter of the meshes. Both protein types show similar distributions with a maximum at ratio of ∼0.7. <b>F</b>: Histograms of the extension of the meshes parallel to the orientation of the whole TJ-network (principal axis) divided by its perpendicular extension showing that the mean orientation of the meshes of both protein types is parallel to the orientation of the whole TJ-network (mean value for Cld3: ∼1.21 with STD: ∼0.38; mean value for Cld5: ∼1.29 with STD: ∼0.42). All histograms are normalized to the total amount of analyzed meshes.