<p>Optical images of ARS staining for mineralization in MG63 osteoblast-like cells for day 28 (A) EC, (B) EG. (C) EC with 10 µM Y27632, (D) EG with 10 µM Y27632. Quantification of mineral deposition by Alizarin Red-S staining. Data represent mean ± SD, n = 4. Statistical analysis was compared between EC and EG with or without Y27632. Different letters represent significance at <i>p</i><0.05 by non-parametric Mann-Whitney U test.</p
<p>Representative images were captured at 100× magnification and the scale bars represent 500 µm. (A...
<p>Primary human osteoblasts cultured in differentiation medium with 5 ng/ml hr TGF-β<sub>1</sub> wi...
<p>Alizarin red staining as well as immunofluorescence demonstrated that there did not occur osteoge...
<p>(A) hASCs used as the negative controls. (B) Image of cells cultured for 7 days, showing a less i...
<p>Alizarin Red S staining of empty scaffolds maintained for 8 weeks in expansion (a) or osteogenic ...
<p>1) no ATRA, no BMP-2/7; 2) 1 µM ATRA, no BMP-2/7; 3) 1 µM ATRA, 5 ng/ml BMP-2/7; 4) 1 µM ATRA, 50...
Alizarin Red-S staining method was used after 144 hours of culture. The concentrations of each drug ...
Alizarin Red S staining is the standard method to indicate and quantify matrix mineralization during...
<p>At time points 5, 7, 10 and 14 days, primary human osteoblasts were fixed and stained with silver...
<p>The panel shows positivity for Collagen I (<b>A</b>) FITC (green) (Calibration Bar = 10 µm), BAP ...
<p>A. results of mineral deposition B. results of ALP activity. Data from cells derived from 7 diffe...
Osteoblasts grown for 3 weeks on different samples in mineralization medium were stained with Alizar...
<p>Only small, diffusely distributed mineral deposits are detected in phase contrast images of stain...
<p>Few mineral nodules could be observed for cells without ES. A greater number of mineral nodules w...
<p>(<b>A</b>) ALP activity performed on day 7 of oAFMSC culture at increased concentrations of R-568...
<p>Representative images were captured at 100× magnification and the scale bars represent 500 µm. (A...
<p>Primary human osteoblasts cultured in differentiation medium with 5 ng/ml hr TGF-β<sub>1</sub> wi...
<p>Alizarin red staining as well as immunofluorescence demonstrated that there did not occur osteoge...
<p>(A) hASCs used as the negative controls. (B) Image of cells cultured for 7 days, showing a less i...
<p>Alizarin Red S staining of empty scaffolds maintained for 8 weeks in expansion (a) or osteogenic ...
<p>1) no ATRA, no BMP-2/7; 2) 1 µM ATRA, no BMP-2/7; 3) 1 µM ATRA, 5 ng/ml BMP-2/7; 4) 1 µM ATRA, 50...
Alizarin Red-S staining method was used after 144 hours of culture. The concentrations of each drug ...
Alizarin Red S staining is the standard method to indicate and quantify matrix mineralization during...
<p>At time points 5, 7, 10 and 14 days, primary human osteoblasts were fixed and stained with silver...
<p>The panel shows positivity for Collagen I (<b>A</b>) FITC (green) (Calibration Bar = 10 µm), BAP ...
<p>A. results of mineral deposition B. results of ALP activity. Data from cells derived from 7 diffe...
Osteoblasts grown for 3 weeks on different samples in mineralization medium were stained with Alizar...
<p>Only small, diffusely distributed mineral deposits are detected in phase contrast images of stain...
<p>Few mineral nodules could be observed for cells without ES. A greater number of mineral nodules w...
<p>(<b>A</b>) ALP activity performed on day 7 of oAFMSC culture at increased concentrations of R-568...
<p>Representative images were captured at 100× magnification and the scale bars represent 500 µm. (A...
<p>Primary human osteoblasts cultured in differentiation medium with 5 ng/ml hr TGF-β<sub>1</sub> wi...
<p>Alizarin red staining as well as immunofluorescence demonstrated that there did not occur osteoge...