Cyclin D1 overexpression rescues miR-520b depressed growth of hepatoma cells.

<p>(A) The effect of miR-520b or sicyclinD1-1 on phosphorylation levels Rb (p-Rb) was examined in HepG2 and H7402 cells by western blot analyses. (B) Western blot analyses showed the expression levels of cyclin D1 and p-Rb in HepG2 and H7402 cells treated by miR-520b or both pcDNA3-cyclin D1 and miR-520b. (C) The effect of cyclin D1 overexpression on the miR-520b-inhibited proliferation of HepG2 cells was examined by flow cytometry analyses. (D) The effect of cyclin D1 overexpression on the miR-520b-inhibited proliferation of HepG2 cells was examined by MTT assays at 24 h, 48 h and 72 h after transfection. *<i>P</i><0.05, ** <i>P</i><0.01, Student's <i>t</i> test.