Location of three IFT27, IFT46 and IFT72 during cleavage furrow formation.

<p>(A,E,I) DIC micrographs of three different dividing Chlamydomonas cells at the time of first cleavage furrow formation. (B,F,J) The immunofluorescence locations of α-tubulin (red), DAPI stained DNA (blue), and the indicated IFT protein (green) observed by widefield microscopy. (B,C) IFT27. (F,G) IFT72. (J,K) IFT46. (D,H,L) 3D reconstructions of optical sections obtained by laser scanning confocal microscopy of three additional dividing Chlamydomonas cells that were subjected to indirect immunofluorescence labeling at the time of cleavage furrow formation. Appearing in red is a reconstruction of each cell's microtubule cytoskeleton shown in side-view at the upper left and right of each panel. The locations of the cleavage furrows (cf) are marked by dotted lines. The 3D reconstructions are rotated about the y-axis giving a view directly into the cleavage furrows in the images shown at the bottom of each panel. The immunofluorescence signals corresponding to IFT27, IFT46 and IFT72 are included and shown as green to the right in panels D, H and L respectively. All three IFT proteins are clustered at the cleavage furrow.