We demonstrate the use of capillary zone electrophoresis with an electrokinetically pumped sheath-flow electrospray interface for the analysis of a tryptic digest of a sample of intermediate protein complexity, the secreted protein fraction of <i>Mycobacterium marinum</i>. For electrophoretic analysis, 11 fractions were generated from the sample using reverse-phase liquid chromatography; each fraction was analyzed by CZE-ESI-MS/MS, and 334 peptides corresponding to 140 proteins were identified in 165 min of mass spectrometer time at 95% confidence (FDR < 0.15%). In comparison, 388 peptides corresponding to 134 proteins were identified in 180 min of mass spectrometer time by triplicate UPLC-ESI-MS/MS analyses, each using 250 ng of the unfrac...
The direct analysis of intact proteins via mass spectrometry offers compelling advantages in compari...
Capillary electrophoresis -- mass spectrometry (CE-MS) is increasingly being used in proteomics rese...
Capillary zone electrophoresis (CZE) with an electrokinetically pumped sheath-flow nanospray interfa...
We demonstrate the use of capillary zone electrophoresis with an electrokinetically pumped sheath-fl...
We demonstrate the use of capillary zone electrophoresis with an electrokinetically pumped sheath-fl...
We demonstrate the use of capillary zone electrophoresis with an electrokinetically pumped sheath-fl...
We demonstrate the use of capillary zone electrophoresis with an electrokinetically pumped sheath-fl...
We demonstrate the use of capillary zone electrophoresis with an electrokinetically pumped sheath-fl...
We demonstrate the use of capillary zone electrophoresis with an electrokinetically pumped sheath-fl...
We demonstrate the use of capillary zone electrophoresis with an electrokinetically pumped sheath-fl...
We demonstrate the use of capillary zone electrophoresis with an electrokinetically pumped sheath-fl...
ABSTRACT: Capillary zone electrophoresis (CZE) with an electrokinetically pumped sheath-flow nanospr...
Capillary zone electrophoresis (CZE)–electrospray ionization-tandem mass spectrometry (ESI-MS/MS) wa...
Untargeted bottom-up proteomic analysis aims to identify the highest number of peptides from complex...
Recent developments in the coupling of liquid phase separations and mass spectrometry have yielded s...
The direct analysis of intact proteins via mass spectrometry offers compelling advantages in compari...
Capillary electrophoresis -- mass spectrometry (CE-MS) is increasingly being used in proteomics rese...
Capillary zone electrophoresis (CZE) with an electrokinetically pumped sheath-flow nanospray interfa...
We demonstrate the use of capillary zone electrophoresis with an electrokinetically pumped sheath-fl...
We demonstrate the use of capillary zone electrophoresis with an electrokinetically pumped sheath-fl...
We demonstrate the use of capillary zone electrophoresis with an electrokinetically pumped sheath-fl...
We demonstrate the use of capillary zone electrophoresis with an electrokinetically pumped sheath-fl...
We demonstrate the use of capillary zone electrophoresis with an electrokinetically pumped sheath-fl...
We demonstrate the use of capillary zone electrophoresis with an electrokinetically pumped sheath-fl...
We demonstrate the use of capillary zone electrophoresis with an electrokinetically pumped sheath-fl...
We demonstrate the use of capillary zone electrophoresis with an electrokinetically pumped sheath-fl...
ABSTRACT: Capillary zone electrophoresis (CZE) with an electrokinetically pumped sheath-flow nanospr...
Capillary zone electrophoresis (CZE)–electrospray ionization-tandem mass spectrometry (ESI-MS/MS) wa...
Untargeted bottom-up proteomic analysis aims to identify the highest number of peptides from complex...
Recent developments in the coupling of liquid phase separations and mass spectrometry have yielded s...
The direct analysis of intact proteins via mass spectrometry offers compelling advantages in compari...
Capillary electrophoresis -- mass spectrometry (CE-MS) is increasingly being used in proteomics rese...
Capillary zone electrophoresis (CZE) with an electrokinetically pumped sheath-flow nanospray interfa...