Growth response to CAPE treatment of PC-3 and PC-3 p21^Cip1 siRNA cells.

<p>Protein levels of wild type PC-3, PC-3 cells transfect with scramble control (20 nM), and PC-3 cells transfected with p21^Cip1 siRNA (20 nM) were determined by Western blotting assay. Proliferation of these PC-3 cells treated with 20 µM CAPE for 24 h was determined by 96-well plate proliferation assay as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031286#s4" target="_blank">Material and Methods</a>.