IL-10 production by B cells from different organs during chronic schistosomiasis and their role in protection against AAI.

<p>(A) WT mice were treated as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030883#pone-0030883-g001" target="_blank">Fig. 1A</a>. Splenic, pulmonary or mesenteric B cells (1×10⁵) were isolated and cultured in the presence of SEA (20 µg/ml) for five days. IL-10 production was measured using ELISA and medium value was subtracted. (B) OVA-sensitized recipient mice received 5×10⁶ B cells from different organs. After challenge, BAL cell numbers and eosinophils were determined (C) BAL numbers and eosinophilia of mice that received 250 µg isotype control or anti-IL-10R abs per mouse one day before the adoptive transfer. (D) The percentage of CD4⁺CD25⁺FoxP3⁺ Treg cells was determined in the lungs of recipient mice. Each graph represents three independent experiments, consisting of five mice per group.