Treg cell induction by IL-10-producing Breg cells.

<p>(A) Irradiated splenic B cells (1×10⁵) were cultured with CD4⁺CD25⁻ T cells (1×10⁵) for 5 days in the presence of anti-CD3 and anti-CD28. Induction of CD4⁺CD25⁺FoxP3⁺ Treg cells (in %) by PBS-uninfected B cells was set at one. Fold change in Treg cell percentage for OVA-uninfected and OVA-infected B cells was calculated. Graph expresses results from three independent experiments. (B) OVA-sensitized DEREG mice were treated as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030883#pone-0030883-g002" target="_blank">Fig. 2B</a> in addition to a DT or PBS injection. This graph expresses two experiments, consisting of five mice per group. (C) WT and IL-10^−/− B cell chimeras were treated as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030883#pone-0030883-g001" target="_blank">Fig. 1A</a>. The MedLNs were collected and the percentage of CD4⁺CD25⁺FoxP3⁺ Treg cells was determined. Figure contains two independent experiments and each group consists of 6 to 8 mice. (D) <i>In vitro</i> co-culture were performed as described in (A) in the presence of blocking anti-IL-10R or isotype control antibodies. Percentage of Treg cells induced in the presence of isotype control was arbitrarily set at 1. Fold change in Treg cell induction in the presence of anti-IL-10R was calculated. Graph represents three independent experiments.