ALK-rearranged lung adenocarcinoma without EML4-ALK and KIF5B-ALK.

<p>Panel A shows the results of anti-ALK immunohistochemistry with the iAEP method on pulmonary adenocarcinoma in situ, nonmucinous. The staining pattern was diffusely cytoplasmic. The basal side of tumor cells was more strongly stained, indicating an uneven subcellular localization of KLC1-ALK protein. FISH analyses revealed that this case was positive in the split assay for <i>ALK</i> (Panel B: individual 5′- and 3′-signals are observed) and negative in <i>EML4-ALK</i> and <i>KIF5B-ALK</i> fusion assays (Panel C: <i>EML4</i>, red; <i>ALK</i>, green; Panel D: <i>KIF5B</i>, green; <i>ALK</i>, red).