Invadopodia structures in CIA have degradation ability.

<p>MDA-MB-231 cells in the CIA assay, showing (A) Cherry-MT1-MMP (red) containing vesicles are delivered to invadopodia structures marked by GFP-N-WASP (green) in CIA. Scale bar 20 µm in the upper panel and 10 µm in the lower panel. (B) DQ collagen is mixed with Matrigel and overlaid on top of the cells in CIA. DQ collagen fluorescence (green) is visualized around some of the actin-rich puncta (blue) with co-localization of N-WASP (red), indicating that they are invadopodia structures in CIA. Scale bar 20 µm.