Differentiation between poorly and highly aggressive human cancer cell lines using automated bioimaging assay.

<p>(<b>A</b>) Scatter plot representation of tumor cell dissemination for indicated prostate (<i>upper</i> graphs), breast (<i>middle</i>), and colorectal cancer cell lines (<i>lower</i> graphs). Number of injected embryos from 2 biological replicates is indicated. (<b>B</b>) MCD determined from data represented in <b>A</b>. Data are presented as mean ± s.e.m. *p<0.05, ***p<0.001. (<b>C</b>) 6 dpi embryo injected with PC3 showing tumor foci burden determined from segmented red channel (<i>left</i>), and represented as scatter plot (<i>right</i>). (<b>D</b>) Automated determination of region for exclusion of tumor foci around implantation site and in area of intestinal development (<i>left</i>), and remaining tumor foci represented as scatter plot (<i>right</i>). (<b>E</b>) MCD before (<i>black</i>) and after exclusion (<i>white bars</i>) for the indicated prostate (<i>left</i>), breast (<i>middle</i>), and colorectal cancer lines (<i>right graph</i>). Fold difference between poorly and highly aggressive cell lines is indicated. Data are presented as mean ± s.e.m. *p<0.05, ***p<0.001. (<b>F</b>) MCD after exclusion for PC3 and MCF7 in multiple independent experiments demonstrates reproducibility. Data are presented as mean ± s.e.m.