Intermolecular interaction of CMM2 domains <i>in vivo.</i>

<p>(A) Radial net representation of the CMM2 sequence with the mutated amino acids of the CMM2-mut1, -mut2 and -mut3 constructs indicated in red, yellow and orange, respectively. On each side of the radial net, the CMM2 coiled-coil structure is shown as green- and orange ribbons with the targeted amino acids displayed as sticks surrounded with a mesh surface (colored as described above). (B) Schematic presentation of vectors used in the yeast two-hybrid experiments (<i>left</i>) and α-galactosidase staining of yeast co-transformed with corresponding protein fusions (right). “X” on the construct models represents approximate positions of mutations (colored according to A). The “empty vector” contained only GAL4-AD and GAL4-BD.