Increased cytolytic activity of CD8⁺ T cells from p210 immunized mice against dendritic cells in vitro.

<p>(A) Representative scatter plot of CD11c-gated cells stained with 7-AAD after co-culture for 4 hours with CD8⁺ T cells from the different experimental groups. CD8⁺ T cells from p210 immunized mice had a significantly higher cytolytic activity against DCs when compared to those from PBS or cBSA/alum groups (B, left panel; N = 11 each; *P<0.05). Depletion of CD25⁺ cells from the CD8⁺ T cells negated the lytic activity specific to p210 immunized mice (B, center panel; N = 4 each). Specificity of the increased lytic activity of CD8⁺ T cells from p210-immunized mice was also negated by performing the assay in media supplemented with 10% delipidated serum (B, right panel; N = 3 each). (C) Dendritic cells were able to uptake p210 as indicated by the presence of green fluorescence in the cell (left panel). When such dendritic cells were co-cultured with CD8⁺ T cells from p210-immunized mice, there was a significant increase of lytic activity when compared to those from PBS or cBSA/alum groups (right panel). Data were analyzed by ANOVA followed by Newman-Keuls multiple group comparison.