Phenotypes related to insect attraction.

<p>(A–D) Base of gynoecia of indicated genotypes. Arrows indicate nectaries. Scale bar, 0.1 mm. (E) Comparative quantitative analyses of floral volatile sesquiterpene emissions from wild-type, <i>myb21-5</i>, <i>myb24-5</i>, and <i>myb21-5 myb24-5</i> mutants. Emitted compounds were collected for 7 h from 40 detached inflorescences by a closed-loop stripping procedure. Emission was determined in ng h⁻¹ per 40 inflorescences. Values are averages and standard deviations of three independent collections. Only emissions of (<i>E</i>)-β-caryophyllene, the product of TPS21, and thujopsene, the product of TPS11, are shown. Different letters indicate significant differences in emissions of each compound between genotypes ( p≤0.001). (F) GC-MS analyses of sesquiterpene hydrocarbons collected via SPME from 20 inflorescences of wild-type, <i>myb21-5</i> and <i>arf6-2 arf8-3</i> mutants. Peaks marked with circles represent sesquiterpenes produced by the terpene synthase TPS21. Compounds not labeled with circles are products of the terpene synthase TPS11, with the exception of α-farnesene (α-farn). 1, (<i>E</i>)-β-caryophyllene; 2, thujopsene; 3, α-humulene; 4, β-chamigrene. Peaks marked with asterisks are other sesquiterpene products of TPS11 or TPS21 <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002506#pgen.1002506-Tholl1" target="_blank">[43]</a>.