Aberrant morphology and compromised function of PGC-1α KO mouse retina under light stress conditions.

<p>PGC-1α <i>KO</i> and C57BL/6j WT, aged 13 weeks, were exposed to 15.000 lux of white light for 2 hours and after 24 hours and 10 days of recovery subjected to morphological and functional analysis, respectively. <b>A.</b> Representative retinal morphologic sections from the lower central retina of PGC-1α KO and C57BL/6j WT mice. <b>B.</b> [<b>A</b>] Scotopic (dark-adapted) and [<b>B</b>] photopic (light-adapted) single-flash ERGs with increasing light intensities recorded from PGC-1α KO and C57BL/6j WT control mice. The vertical line crossing each trace shows the time point of the light flash. [<b>C</b>] Scotopic and [<b>D</b>] photopic b-wave amplitudes from PGC-1α KO and C57BL/6 control mice as a function of the logarithm of the flash intensity. (<b>c</b>) Optical coherence tomography (SD-OCT): [<b>A</b>] comparison of two representative OCT horizontal slices of PGC-1α KO and C57BL/6j WT control mice. [<b>B</b>] Magnification of these SD-OCT horizontal slices: left side: C57BL/6, right side: PGC-1α KO; GC = ganglion cell layer, IPL = inner plexiform layer, INL = inner nuclear layer, OPL = outer plexiform layer, ONL = outer nuclear layer, OLM = outer limiting membrane, IS/OS = inner segment/outer segment, RPE = retinal pigment epithelium [<b>C</b>] Topography of retinal thickness of C57BL/6 (side) and PGC-1α KO (right) mice calculated from 61 equidistant SD-OCT slices (green = dense; blue = less dense).