Direct evidence for Aβ_1–42 binding <i>in vitro</i>.

<p>(A–C) Curcumin degradation at neutral pH (PBS) solution over time assessed by absorbance at 440 nm for solutions containing 0.0001% (yellow), 0.001% (orange), and 0.01% (red) (w/v) curcumin. Solid lines and dotted lines represented in presence and in absence of 10 µM Aβ_1–42 peptide respectively. Black lines represents background absorbance of buffer (dotted line) and 10 µM Aβ_1–42 peptide (solid line) in the absence of curcumin. (D–F) Curcumin absorbance spectra for the corresponding curcumin containing samples as those described in A–C at 0 h, 2 hours and 10 hours of incubation colored in gray scale.