Adhesion complex turnover in ROCK1- and ROCK2-depleted keratinocytes.

<p>Focal adhesion size and dynamics were analysed in HaCaT-NSC, HaCaT-ROCK1-KD and HaCaT-ROCK2-KD cells transiently transfected with mRFP-paxillin. Live images of adhesion complexes were taken every 20 seconds for 20 minutes and representative images are shown (A–C). Rates of adhesion complex assembly (D) and disassembly (E) were calculated. A minimum of 12 cells per population was analysed in each experiment and the data are the mean and standard error from 3 separate experiments. Statistical analysis was performed using a Mann Whitney test (**p<0.01 *p<0.05). See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031423#pone.0031423.s003" target="_blank">Videos S1</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031423#pone.0031423.s004" target="_blank">S2</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031423#pone.0031423.s005" target="_blank">S3</a> for representative movies).