<p>HeLa cells were transfected with constructs encoding the indicated proteins. At 16–18 hours post-transfection, cells were processed for immunofluorescence microscopy and immunostained for α-tubulin. DAPI was used to visualise the nuclei. Images were acquired by confocal microscopy. Scale bar indicates 10 µm. Data are typical of at least three independent experiments.</p
<p>LHCN-M2 cells were transfected with a DUX4c or DUX4 expression vector (<i>p-DUX4</i>, <i>p-DUX4c<...
(A) Ultrastructural analysis. HeLa cells were co-transfected with plasmids encoding the indicated pr...
<p>HeLa cells were synchronized as described in the legend of Fig. 2, and samples of cells were then...
<p>(<b><i>A</i></b> and <b><i>B</i></b>) HeLa cells were transfected with constructs encoding the in...
<p>HeLa cells were transfected with constructs encoding the indicated proteins. At 36–40 hours post-...
<p>(A) Subcellular fractionation of IMS32 cell lysates was conducted by discontinuous sucrose densit...
<p><b>A</b> and <b>B</b>, Western blots showed that the expression of MAP4 and gross quantity of α-t...
<p>(<b><i>A</i></b>) Yeast two-hybrid analysis of the interaction between the indicated proteins. Pr...
<p>Plasmids pEGFP-N1 (Control, left panels), pEGFP-N1-Ilf3/NF90 common N-terminal short sequence (Sh...
(A) Potential pUs3 substrates, indicated in red, reside in HSV-2 pUL31 (top) and in HSV-2 pUL34 (bot...
<p>(A) Murine lung fibroblasts (WT ACTN4 and ACTN4 KD) were transiently transfected to express the d...
<p>(<b>A</b>) Forty eight hours after transfection, proteins were extracted from NSC-34 cells and th...
<p>293T cells were transfected either with Gag WT (upper panels), Gag min (middle panels) or pcDNA3....
<p>(A) Co-labelling of myc-tagged proteins (green) and tubulin (red) on isolated flagella from WT ce...
<p>LHCN-M2 cells were transfected with a DUX4c or DUX4 expression vector (<i>p-DUX4</i>, <i>p-DUX4c<...
<p>LHCN-M2 cells were transfected with a DUX4c or DUX4 expression vector (<i>p-DUX4</i>, <i>p-DUX4c<...
(A) Ultrastructural analysis. HeLa cells were co-transfected with plasmids encoding the indicated pr...
<p>HeLa cells were synchronized as described in the legend of Fig. 2, and samples of cells were then...
<p>(<b><i>A</i></b> and <b><i>B</i></b>) HeLa cells were transfected with constructs encoding the in...
<p>HeLa cells were transfected with constructs encoding the indicated proteins. At 36–40 hours post-...
<p>(A) Subcellular fractionation of IMS32 cell lysates was conducted by discontinuous sucrose densit...
<p><b>A</b> and <b>B</b>, Western blots showed that the expression of MAP4 and gross quantity of α-t...
<p>(<b><i>A</i></b>) Yeast two-hybrid analysis of the interaction between the indicated proteins. Pr...
<p>Plasmids pEGFP-N1 (Control, left panels), pEGFP-N1-Ilf3/NF90 common N-terminal short sequence (Sh...
(A) Potential pUs3 substrates, indicated in red, reside in HSV-2 pUL31 (top) and in HSV-2 pUL34 (bot...
<p>(A) Murine lung fibroblasts (WT ACTN4 and ACTN4 KD) were transiently transfected to express the d...
<p>(<b>A</b>) Forty eight hours after transfection, proteins were extracted from NSC-34 cells and th...
<p>293T cells were transfected either with Gag WT (upper panels), Gag min (middle panels) or pcDNA3....
<p>(A) Co-labelling of myc-tagged proteins (green) and tubulin (red) on isolated flagella from WT ce...
<p>LHCN-M2 cells were transfected with a DUX4c or DUX4 expression vector (<i>p-DUX4</i>, <i>p-DUX4c<...
<p>LHCN-M2 cells were transfected with a DUX4c or DUX4 expression vector (<i>p-DUX4</i>, <i>p-DUX4c<...
(A) Ultrastructural analysis. HeLa cells were co-transfected with plasmids encoding the indicated pr...
<p>HeLa cells were synchronized as described in the legend of Fig. 2, and samples of cells were then...