Characterization of TNPs from BM and blood.

<p>Lineage depleted BM and blood cells from C57BL/6 mice were stained for lineage markers, CD27, CD135, CD117, CD127 and CD90. A) Gating strategy for the identification of TNPs in BM and blood. Representative data from 1 out of 4 mice (BM) and pooled cells from 10 mice (blood). B) Relative quantification of MPPs (lin⁻CD27⁺CD135⁺CD127⁻CD117^hi), CLPs (lin⁻CD27⁺CD135⁺CD127⁺CD117^+/low), TNPs (lin⁻CD27⁺CD135⁺CD127⁻CD117^−/lowCD90⁻) and CD90⁺ (lin⁻CD27⁺CD135⁺CD127⁻CD117^−/lowCD90⁺) candidate T cell precursors within lin⁻CD27⁺CD135⁺ cells. Combined data of 4 mice and 20 mice for BM and blood, respectively, from 2 independent experiments. Error bars indicate SEM.